Optical imaging is crucial for life science to study, understand and reveal new structures or phenomena. Recently, multi-cellular aggregates that mimics in vitro and in 3D some functions of organs (organoids) emerged as new biological models. Even if remarkable efforts have been made to adapt existing tools to image developing multicellular aggregates, current solutions are insufficient to observe the spatiotemporal organization of such samples over days or weeks in non-invasive manner.
My objective is to develop new imaging techniques or modalities dedicated to the non-invaisve observation of organoids.
In the BiOf lab, we aim to develop simple, robust and cost-effective solutions to image large populations of multi-cellular aggregates (organoids) placed directly inside an incubator.
We recently developped an augmented variant of confocal microscopy where the key innovation consists to use a series of reflecting pinholes axially distributed in the detection plane, each one probing a different depth within the sample.
Light-field microscopy (LFM) offers instantaneous 3D imaging by capturing both spatial and angular information from a semi-transparent specimen. We aim to push back the current limits of LFM both in terms of depth and imaging volumes by combining innovative experimental and numerical approaches.
Postdoctoral scientists :
PhD students :