Amaury Badon

CNRS researcher

Research interests

Optical imaging is crucial for life science to study, understand and reveal new structures or phenomena. Recently, multi-cellular aggregates that mimics in vitro  and in 3D some functions of organs (organoids) emerged as new biological models. Even if remarkable efforts have been made to adapt existing tools to image developing multicellular aggregates, current solutions are insufficient to observe the spatiotemporal organization of such samples over days or weeks in non-invasive manner.

My objective is to develop new imaging techniques or modalities dedicated to the non-invaisve observation of organoids.

Large-scale label-free microscopy

In the BiOf lab, we aim to develop simple, robust and cost-effective solutions to image large populations of multi-cellular aggregates (organoids) placed directly inside an incubator.

Capture

Full-field optical coherence tomography (FF-OCT)

Full-field OCT is a simple interferometric technique that provides rapid and high-resolution en-face images of biolofical samples. We aim to develop new techniques to increase both the imaging depth, contrast and field of view of FF-OCT.

Multiplane confocal microscopy

We recently developped an augmented variant of confocal microscopy where the key innovation consists to use a series of reflecting pinholes axially distributed in the detection plane, each one probing a different depth within the sample.

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Light-field microscopy

Light-field microscopy (LFM) offers instantaneous 3D imaging by capturing both spatial and angular information from a semi-transparent specimen. We aim to push back the current limits of LFM both in terms of depth and imaging volumes by combining innovative experimental and numerical approaches.

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Professional experience

CNRS researcher – (2021-present)

Label-free imaging of organoids

Laboratoire Photonique Numérique & Nanosciences, CNRS & University of Bordeaux, Bordeaux, FR

 

Post-doctoral  scientist – (2019-2021)

Label-free imaging of organoids

Laboratoire Photonique Numérique & Nanosciences, CNRS & University of Bordeaux, Bordeaux, FR

Advisor: Pierre Nassoy

 

Post-doctoral  scientist – (2017-2019)

Multi-plane confocal microscopy

College of Engineering, Boston University, Boston, USA

Advisor: Jerome Mertz

 

PhD in Optics – 2013-2016

Propagation of light in complex media

Institut Langevin, Paris, France

Supervisor: Alexandre Aubry

Education

PhD in Optics – (2013-2016)

Institut Langevin – ESPCI – Paris

 

Master 2 (DEA) in Condensed Matter Physics – 2013

ENS Paris – Université Paris Sud

 

Engineering school in Physics and Chemistry  (2009-2012)

ESPCI Paris