(P. Nassoy with TreeFrog Therapeutics)
Encapsulation of human induced pluripotent stem cells (hIPSC) inside alginate capsules qithe the appropriate niche-like environment allows the formation of lumenized three-dimensional colonies that significantly improve viabilityand expansion rates as compared with standard hPSC. We are now investigating in details tha growth, organization and mechanical properties of these stem cell cysts.
Cohen, P. J., Luquet, E., Pletenka, J., Leonard, A., Warter, E., Gurchenkov, B., …Nassoy, P., … & Alessandri, K. (2023). Engineering 3D micro-compartments for highly efficient and scale-independent expansion of human pluripotent stem cells in bioreactors. Biomaterials, 122033.